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Here, we assessed the feasibility of greater occipital nerve (GON) tractography using a fully automated tractography technique on the whole-neck volume, in comparison with anatomical knowledge. GON complete visualization (from C1–C2 origin to muscular emergence) was possible in 18 out of 20 healthy subjects using a 32 diffusion-weighted directions MR sequence (A) with Track Weighted-Imaging super-resolution global tractography process (B). It is also possible to extract both GON with overlaying onto anatomical sequences (C and D). This technique may appear as a promising technique for therapeutic management of patients with occipital neuralgia.